ChloroquineV1, France, Analysis, bibRecord, 000313

A non-radiolabeled heme–GSH interaction test for the screening of antimalarial compounds

Identifieur interne : 000313 ( France/Analysis ); précédent : 000312; suivant : 000314

A non-radiolabeled heme–GSH interaction test for the screening of antimalarial compounds

Auteurs : Giovanny Garavito [Colombie] ; Marie-Carmen Monje [France] ; Séverine Maurel [France] ; Alexis Valentin [France] ; Françoise Nepveu [France] ; Eric Deharo [France]

Source :

Experimental Parasitology [ 0014-4894 ] ; 2007-01-23.

RBID : Hal:hal-02143188

English descriptors

mix :
- Amodiaquine, Antimalarials, Arthemether, Atovaquone, Chloroquine, Glutathione, Heme, High performance liquid chromatography, Mefloquine, Methylene blue, Plasmodium falciparum, Quinine, Reduced glutathione- O-phthalaldehyde, Screening.

Abstract

Intraerythrocytic Plasmodium produces large amounts of toxic heme during the digestion of hemoglobin, a parasite specific pathway. Heme is then partially biocristallized into hemozoin and mostly detoxified by reduced glutathione. We proposed an in vitro micro assay to test the ability of drugs to inhibit heme-glutathione dependent degradation. As glutathione and o-phthalaldehyde form a fluorescent adduct, we followed the extinction of the fluorescent signal when heme was added with or without antimalarial compounds. In this assay, 50 microM of amodiaquine, arthemether, chloroquine, methylene blue, mefloquine and quinine inhibited the interaction between glutathione (50 microM) and heme (50 microM), while atovaquone did not. Consequently, this test could detect drugs that can inhibit heme-GSH degradation in a fast, simple and specific way, making it suitable for high throughput screening of potential antimalarials.

Url:

https://hal.archives-ouvertes.fr/hal-02143188

DOI: 10.1016/j.exppara.2007.01.005

Affiliations:

Colombie, France

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000007
to stream Hal, to step Curation: 000007
to stream Hal, to step Checkpoint: 000226
to stream Main, to step Merge: 001A62
to stream Main, to step Curation: 001A52
to stream Main, to step Exploration: 001A52
to stream France, to step Extraction: 000313

Links to Exploration step

Hal:hal-02143188

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A non-radiolabeled heme–GSH interaction test for the screening of antimalarial compounds</title>
<author><name sortKey="Garavito, Giovanny" sort="Garavito, Giovanny" uniqKey="Garavito G" first="Giovanny" last="Garavito">Giovanny Garavito</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-323000" status="VALID">          <orgName>Universidad Nacional de Colombia [Bogotà]</orgName>
          <orgName type="acronym">UNAL</orgName>
          <desc>            <address>              <addrLine>Cra 45, Bogotá, Colombie</addrLine>
              <country key="CO"></country>
            </address>
            <ref type="url">http://unal.edu.co/</ref>
          </desc>
        </hal:affiliation>
<country>Colombie</country>
</affiliation>
</author>
<author><name sortKey="Monje, Marie Carmen" sort="Monje, Marie Carmen" uniqKey="Monje M" first="Marie-Carmen" last="Monje">Marie-Carmen Monje</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Maurel, Severine" sort="Maurel, Severine" uniqKey="Maurel S" first="Séverine" last="Maurel">Séverine Maurel</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Valentin, Alexis" sort="Valentin, Alexis" uniqKey="Valentin A" first="Alexis" last="Valentin">Alexis Valentin</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Nepveu, Francoise" sort="Nepveu, Francoise" uniqKey="Nepveu F" first="Françoise" last="Nepveu">Françoise Nepveu</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Deharo, Eric" sort="Deharo, Eric" uniqKey="Deharo E" first="Eric" last="Deharo">Eric Deharo</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-02143188</idno>
<idno type="halId">hal-02143188</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-02143188</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-02143188</idno>
<idno type="doi">10.1016/j.exppara.2007.01.005</idno>
<date when="2007-01-23">2007-01-23</date>
<idno type="wicri:Area/Hal/Corpus">000007</idno>
<idno type="wicri:Area/Hal/Curation">000007</idno>
<idno type="wicri:Area/Hal/Checkpoint">000226</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000226</idno>
<idno type="wicri:doubleKey">0014-4894:2007:Garavito G:a:non:radiolabeled</idno>
<idno type="wicri:Area/Main/Merge">001A62</idno>
<idno type="wicri:Area/Main/Curation">001A52</idno>
<idno type="wicri:Area/Main/Exploration">001A52</idno>
<idno type="wicri:Area/France/Extraction">000313</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A non-radiolabeled heme–GSH interaction test for the screening of antimalarial compounds</title>
<author><name sortKey="Garavito, Giovanny" sort="Garavito, Giovanny" uniqKey="Garavito G" first="Giovanny" last="Garavito">Giovanny Garavito</name>
<affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-323000" status="VALID">          <orgName>Universidad Nacional de Colombia [Bogotà]</orgName>
          <orgName type="acronym">UNAL</orgName>
          <desc>            <address>              <addrLine>Cra 45, Bogotá, Colombie</addrLine>
              <country key="CO"></country>
            </address>
            <ref type="url">http://unal.edu.co/</ref>
          </desc>
        </hal:affiliation>
<country>Colombie</country>
</affiliation>
</author>
<author><name sortKey="Monje, Marie Carmen" sort="Monje, Marie Carmen" uniqKey="Monje M" first="Marie-Carmen" last="Monje">Marie-Carmen Monje</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Maurel, Severine" sort="Maurel, Severine" uniqKey="Maurel S" first="Séverine" last="Maurel">Séverine Maurel</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Valentin, Alexis" sort="Valentin, Alexis" uniqKey="Valentin A" first="Alexis" last="Valentin">Alexis Valentin</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Nepveu, Francoise" sort="Nepveu, Francoise" uniqKey="Nepveu F" first="Françoise" last="Nepveu">Françoise Nepveu</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Deharo, Eric" sort="Deharo, Eric" uniqKey="Deharo E" first="Eric" last="Deharo">Eric Deharo</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-192672" status="VALID">          <idno type="ISNI">0000 0004 0490 5384</idno>
          <idno type="RNSR">200317398C</idno>
          <orgName>Pharmacochimie et Biologie pour le Développement</orgName>
          <orgName type="acronym">PHARMA-DEV</orgName>
          <date type="start">2003-01-01</date>
          <desc>            <address>              <addrLine>IRD UMR 152 Université Paul Sabatier 35 Chemin des Maraîchers 31400 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.pharmadev.ird.fr/</ref>
          </desc>
          <listRelation>            <relation name="UMR152" active="#struct-67872" type="direct"></relation>
            <relation active="#struct-159253" type="direct"></relation>
            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="indirect"></relation>
            <relation active="#struct-448187" type="indirect"></relation>
          </listRelation>
        <tutelles><tutelle name="UMR152" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-159253" type="direct"><org type="regrouplaboratory" xml:id="struct-159253" status="VALID">          <idno type="IdRef">240309472</idno>
          <idno type="ISNI">0000 0004 4686 8315</idno>
          <idno type="RNSR">200310667L</idno>
          <orgName>Institut de Chimie de Toulouse</orgName>
          <orgName type="acronym">ICT-FR 2599</orgName>
          <desc>            <address>              <addrLine>118 Route de Narbonne 31062 Toulouse cédex 9</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://ict.ups-tlse.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-67872" type="direct"></relation>
            <relation active="#struct-217752" type="direct"></relation>
            <relation active="#struct-443875" type="indirect"></relation>
            <relation name="FR2599" active="#struct-441569" type="direct"></relation>
            <relation active="#struct-448187" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" status="VALID">          <orgName>Institut de Recherche pour le Développement</orgName>
          <orgName type="acronym">IRD</orgName>
          <desc>            <address>              <addrLine>SiègeLe Sextant 44, bd de DunkerqueCS 9000913572 Marseille cedex 02</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.ird.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-217752" type="direct"><org type="institution" xml:id="struct-217752" status="VALID">          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <desc>            <address>              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
<tutelle active="#struct-443875" type="indirect"><org type="regroupinstitution" xml:id="struct-443875" status="VALID">          <orgName>Université Fédérale Toulouse Midi-Pyrénées</orgName>
          <desc>            <address>              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"></country>
            </address>
          </desc>
        </org>
</tutelle>
<tutelle name="FR2599" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>            <address>              <country key="FR"></country>
            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
        </org>
</tutelle>
<tutelle active="#struct-448187" type="indirect"><org type="institution" xml:id="struct-448187" status="VALID">          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>            <address>              <country key="FR"></country>
            </address>
          </desc>
          <listRelation>            <relation active="#struct-443875" type="direct"></relation>
          </listRelation>
        </org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1016/j.exppara.2007.01.005</idno>
<series><title level="j">Experimental Parasitology</title>
<idno type="ISSN">0014-4894</idno>
<imprint><date type="datePub">2007-01-23</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Amodiaquine</term>
<term>Antimalarials</term>
<term>Arthemether</term>
<term>Atovaquone</term>
<term>Chloroquine</term>
<term>Glutathione</term>
<term>Heme</term>
<term>High performance liquid chromatography</term>
<term>Mefloquine</term>
<term>Methylene blue</term>
<term>Plasmodium falciparum</term>
<term>Quinine</term>
<term>Reduced glutathione- O-phthalaldehyde</term>
<term>Screening</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>Intraerythrocytic Plasmodium produces large amounts of toxic heme during the digestion of hemoglobin, a parasite specific pathway. Heme is then partially biocristallized into hemozoin and mostly detoxified by reduced glutathione. We proposed an in vitro micro assay to test the ability of drugs to inhibit heme-glutathione dependent degradation. As glutathione and o-phthalaldehyde form a fluorescent adduct, we followed the extinction of the fluorescent signal when heme was added with or without antimalarial compounds. In this assay, 50 microM of amodiaquine, arthemether, chloroquine, methylene blue, mefloquine and quinine inhibited the interaction between glutathione (50 microM) and heme (50 microM), while atovaquone did not. Consequently, this test could detect drugs that can inhibit heme-GSH degradation in a fast, simple and specific way, making it suitable for high throughput screening of potential antimalarials.</p>
            </div>
</front>
</TEI>
<affiliations><list><country><li>Colombie</li>
<li>France</li>
</country>
</list>
<tree><country name="Colombie"><noRegion><name sortKey="Garavito, Giovanny" sort="Garavito, Giovanny" uniqKey="Garavito G" first="Giovanny" last="Garavito">Giovanny Garavito</name>
</noRegion>
</country>
<country name="France"><noRegion><name sortKey="Monje, Marie Carmen" sort="Monje, Marie Carmen" uniqKey="Monje M" first="Marie-Carmen" last="Monje">Marie-Carmen Monje</name>
</noRegion>
<name sortKey="Deharo, Eric" sort="Deharo, Eric" uniqKey="Deharo E" first="Eric" last="Deharo">Eric Deharo</name>
<name sortKey="Maurel, Severine" sort="Maurel, Severine" uniqKey="Maurel S" first="Séverine" last="Maurel">Séverine Maurel</name>
<name sortKey="Nepveu, Francoise" sort="Nepveu, Francoise" uniqKey="Nepveu F" first="Françoise" last="Nepveu">Françoise Nepveu</name>
<name sortKey="Valentin, Alexis" sort="Valentin, Alexis" uniqKey="Valentin A" first="Alexis" last="Valentin">Alexis Valentin</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/France/Analysis

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000313 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000313 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    ChloroquineV1
   |flux=    France
   |étape=   Analysis
   |type=    RBID
   |clé=     Hal:hal-02143188
   |texte=   A non-radiolabeled heme–GSH interaction test for the screening of antimalarial compounds
}}

This area was generated with Dilib version V0.6.33.
Data generation: Wed Mar 25 22:43:59 2020. Site generation: Sun Jan 31 12:44:45 2021

	Serveur d'exploration Chloroquine
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration Chloroquine

A non-radiolabeled heme–GSH interaction test for the screening of antimalarial compounds

A non-radiolabeled heme–GSH interaction test for the screening of antimalarial compounds

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri